Request QuoteCatalog Number: xP427518AUQSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP427518AUQYeast1mgQuote
EP427518AUQE. coli1mgQuote
BP427518AUQBaculovirus200ugQuote
MP427518AUQMammalian Cell200ugQuote

Protein Information

SpeciesAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs) )
UniProt IDA8MFD5
Gene NamemiaB; Locus:Clos_1555
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-471
Expression Tag6xHis
Purity>90%
AA SequenceMSKRAKVVVSPEKIKKQSDIIEELKIYNRGKNKKYMITTYGCQMNEHDSETLSGMLENMG YSITTNKEEANLIIYNTCCVRENAELKVYGNIGALKALKKKNEDLIIAVCGCMMQQPQVV KEIKRKYRHVDLVFGTHNLYRFPELLSRSMETEGMFIEVWDEETGIVEGLPANRKYDLKG FINIMYGCNNFCTYCIVPYTRGRERSREVADIIREATDLANNGTKEITLLGQNVNSYGKT LEHPIDFADLLRALNKIDGIERIRFMTSHPKDLSERLIDAIAECDKVCEHFHLPFQSGSN QILKAMNRKYTKENYLSIVKKLKDRIPNIGLTTDIIVGFPGETEEDFQDTLDIVQEARYD SAYTFLYSIREGTPAAKMQNQIDEKVKQERFSRLLDKVNEISAEINQSYLNKVVEVLVEG PSKTDSNKLMGRTRQNKLVNFSGDESLIGKLVNVRIVECRTFSLNGEVIQE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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