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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP414874CWEYeast1mgQuote
EP414874CWEE. coli1mgQuote
BP414874CWEBaculovirus200ugQuote
MP414874CWEMammalian Cell200ugQuote

Protein Information

SpeciesCampylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97)
UniProt IDA7H4S5
Gene NamemiaB; Locus:JJD26997_1481
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-433
Expression Tag6xHis
Purity>90%
AA SequenceMSAKKLFIQTLGCAMNVRDSEHMIAELTQKENYALTEDIKEADLILINTCSVREKPVHKL FSEVGGFEKVKKEGAKIGVCGCTASHLGNEIFKRAPYVDFVLGARNISKITQAIKTPKFM GIDIDYDESEFAFADFRNSIYKSYINISIGCDKHCTYCIVPHTRGDEISIPFNIIHKEAQ KAVEKGAKEIFLLGQNVNNYGKRFRNEHKKMDFSDLLEELSTIEDLERIRFTSPHPLHMD DKFLEVFANNPKVCKSMHMPLQSGSSEILKAMKRGYTKKWYLNRALKLRELCPNVSISTD IIVAFPGESEKDFEETMDVLEKVRFEQIFSFKYSKRPLTKAATMSNQIDEETASRRLSTL QNRHSEILDEIVKKQENKTFKVLFEELRVGNSIAGRTDNNFLVQVEGSEELLGQFKEVKI TNAKRMVLYGEII
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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