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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP411154AUGYeast1mgQuote
EP411154AUGE. coli1mgQuote
BP411154AUGBaculovirus200ugQuote
MP411154AUGMammalian Cell200ugQuote

Protein Information

SpeciesActinobacillus succinogenes (strain ATCC 55618 / 130Z)
UniProt IDA6VQQ3
Gene NamemiaB; Locus:Asuc_1952
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTQKLHIKTWGCQMNEYDSAKIADLLLSTHGLELTEEAEQADVLLLNTCSIREKAQEKVF SQLGRWKTWKNEKPGLIIGVGGCVASQEGEHIRERAPFVDIIFGPQTLHRLPEMINQIRG GKSSVVDVSFPEIEKFDCLPEPKAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPLDD VLFEVAQLADQGVREINLLGQNVNAYRGPTHDGGICTFAELLRLVASIDGIDRLRFTTSH PIEFTDDIIDVYADTPELVSFLHLPVQSGADRILTMMKRGHTALEYKSIIRKLRKVRPDI QISSDFIVGFPGETNEEFEQTMNLIADVNFDMSFSFVYSARPGTPAADMPDDVSEEEKKQ RLYLLQQRINNQAAKFSRAMLGTEQRVLVEGPSKKDIMELTGRTENNRIVNFKGTPDMIG KFVDIQITDVFTNSLRGDVIRTEDEMGLRVEQSPQSVIRRTRKEDELGVGKYVA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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