Request QuoteCatalog Number: xP531717MTESize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP531717MTEYeast1mgQuote
EP531717MTEE. coli1mgQuote
BP531717MTEBaculovirus200ugQuote
MP531717MTEMammalian Cell200ugQuote

Protein Information

SpeciesMethylobacterium sp. (strain 4-46)
UniProt IDB0UNY0
Gene NamemiaB; Locus:M446_3182
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-447
Expression Tag6xHis
Purity>90%
AA SequenceMKKAFVKSYGCQMNVYDAARMVDLLGREGFAETEAMEEADVVILNTCHIREKAAEKVYSE LGRVRDLKGERAGAGRETTIVVAGCVAQAEGREIIHRAPAVDVVVGPQSYHRLPELLARA RDGKVVDTEFPLDDKFDHLPPRRVAGPSAFLTVQEGCDKFCAFCVVPYTRGAEVSRPVAK ILAEAERLAAGGARELTLIGQNVNAFHGEGPDGRPWSLGRLMRRLAEVPGIARLRYTTSH PRDMDDDLIAAHRDCPAVMPYLHLPVQSGSDRILAAMNRRHDADTYRRLIDRIRAARPDI ALTSDFIVGFPGETDADHAETMRLVAEIGFSGAFSFKYSPRPGTPAAESADAVPEAVKRE RLAALQALIDGQARAFNLASLGRTVEVLIEKPGRHPGQVAGKSPYLQAVQIEAEPAVIGT ILPVRLTRAGSNSLFGERAEAAPAAAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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