Request QuoteCatalog Number: xP429838RIHSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP429838RIHYeast1mgQuote
EP429838RIHE. coli1mgQuote
BP429838RIHBaculovirus200ugQuote
MP429838RIHMammalian Cell200ugQuote

Protein Information

SpeciesRenibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235)
UniProt IDA9WQ47
Gene NamemiaB; Locus:RSal33209_0726
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMTLTVSPQESAHQQDESVTPRTYEVRTYGCQMNVHDSERMAGLLERSGLVKAEGKVADVV VFNTCAVRENADNKLYGNLGMLAPAKEANPGMQIAVGGCLAQKDRDTILKRAPWVDVVFG THNVGALPTLLERARHNEEAQLEILESLDVFPSTLPTKRDAVYSGWVSISVGCNNTCTFC IVPSLRGKEKDRRPGDILAEIQALVEDGAIEVTLLGQNVNSYGVEFGDRQAFSKLLRACG EIEGLERVRFTSPHPAAFTEDVIEAMAETHNVMPQLHMPLQSGSDKVLKDMRRSYRSSKF LGILDKVRTQIPDAAISTDIIVGFPGETEEDFQATLDVVAKSRFATAFTFQYSKRPGTPA ADLPDQLPKAVVQERFERLTALQDQISAEENARQIGREVELMVTAGSGKKAAETQRLSGR AKDSRLVHFAVPEGEETPRPGDLVTVTITEAASFHLVADPLPGSVYRLRRSRAGDAWDLA QAEFCGVPNGAQGTSDGGKSSVSLGMPTIPVLPAR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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