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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP534250DUJYeast1mgQuote
EP534250DUJE. coli1mgQuote
BP534250DUJBaculovirus200ugQuote
MP534250DUJMammalian Cell200ugQuote

Protein Information

SpeciesClostridium botulinum (strain Okra / Type B1)
UniProt IDB1IM69
Gene NamemiaB; Locus:CLD_2839
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMNEILNTKDINAIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCC VRENAELKVYGNLGILKGLKSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNL HNFPNYLNEVKKKDTSVLKIQEKEDSIIENMPIDRKNSMKAFVTIMYGCNNFCTYCIVPY VRGRERSRTPENIEDEIKKLISEGYKEITLLGQNVNSYGKDLEPKVTFAELLERVNTIDG LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVV SKIKKLIPNVALSTDIIVGFPGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFE DQVPEDVKHKRFNRLVEVVNEISAKKNKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLV NFIGDKDSIGKLVNVKIIKANSFSLTGEEI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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