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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP407663AUPYeast1mgQuote
EP407663AUPE. coli1mgQuote
BP407663AUPBaculovirus200ugQuote
MP407663AUPMammalian Cell200ugQuote

Protein Information

SpeciesAlkaliphilus metalliredigens (strain QYMF)
UniProt IDA6TR80
Gene NamemiaB; Locus:Amet_2545
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-476
Expression Tag6xHis
Purity>90%
AA SequenceMSKREEVKISEEDLNKQREIINKLREINDAEYERTGKRKKHLTVTYGCQMNEHDSEKLAG ILQNIGYIETNDKNEANLIIYNTCCVRENAELKVYGNIGSLKNLKKEKHDLTIAVCGCMM QQPHVVKEIKSKYRHVDLVFGTHNLHKFPELLSTSMESDNMIIDVWDKEGDIVEGLPVTR KYGLKAFINIMFGCNNFCTYCIVPHTRGRERSREVQEIIDEVEELAKNGTKEITLLGQNV NSYGKTLEEETDFGNLLKVLNKIEGIERIRFMTSHPKDLSDSLISAMTVCTKVCAHLHLP FQAGSNDILKAMNRKYTKEAYLELVEKVRSKVPNVALTTDIIVGFPGETEDDFKETLNVV EKARFNSAYTYLYSLRQETPAAKMENHVPDDVKHERFNRLIEAVNRISGEINKAYLNKVV EVLVEGTSKTDDAKLMGRTPQSKLVNFAGNKEDIGQIVRVKITEPKTFSLNGELLK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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