Request QuoteCatalog Number: xP433546DHVSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP433546DHVYeast1mgQuote
EP433546DHVE. coli1mgQuote
BP433546DHVBaculovirus200ugQuote
MP433546DHVMammalian Cell200ugQuote

Protein Information

SpeciesDelftia acidovorans (strain DSM 14801 / SPH-1)
UniProt IDA9BUQ1
Gene NamemiaB; Locus:Daci_1515
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMSKKVFIKTFGCQMNEYDSDKMADVLGAAQGYESTDDPEQADLILFNTCSVREKAQEKVF SDLGRYKHLKERGVLIGVGGCVASQEGEEIIKRAPYVDVVFGPQTLHRLPELLNARAAKH KPQVDISFPEIEKFDHLPPARVEGSSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDVL IEVAGLADQGVKEVTLLGQNVNAYLGKMGDTAEIADFALLLEYVSEIPGIERIRYTTSHP NEFTPRLIEAYARLPKLVSHLHLPVQHGSDKILMAMKRGYTAMEYKSTIRKLRAIRPEMA MSSDFIVGFPGETEEDFQKMMKLIHDVRFDNSFSFIFSPRPGTPAANLHDDTPHEVKLRR LQELQAVINNNIKDISDERVGTVQRLLVEGLSKRDGSELMGRTECNRVVNFPGNERLIGQ MIDVKITEARTFTLRGEVVVRH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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