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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP430880PYIYeast1mgQuote
EP430880PYIE. coli1mgQuote
BP430880PYIBaculovirus200ugQuote
MP430880PYIMammalian Cell200ugQuote

Protein Information

SpeciesPetrotoga mobilis (strain DSM 10674 / SJ95)
UniProt IDA9BGV7
Gene NamemiaB; Locus:Pmob_1849
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-439
Expression Tag6xHis
Purity>90%
AA SequenceMKFYIRTFGCQMNINESEIMAGLLKEEGFEWTENPKEADIILINSCAVREKAENKMYGAI GGYGKLKDENKNLILGVGGCSAEKERENLLERFKNIDFVFGTRNVVDIGNLVKRALNGKR FADFSDKLNDVNYDIPKMPISKHHAWITIIYGCNKYCSYCIVPYTRGFEKSRPMEDIIRE VESYAKKGYKEITFLGQNVDSYGKDFGDKKSKLDLLIQKAAEFDSIKRIWFLTSYPSDIT DSLIQTVANEEKAANYFHLPAQSGSNKILKAMNRKYTREEFIELVNKVKKEVANVTVSSD FITGFPSETDEDFEETVDLIKQCRFERINIAEYSPREGTIAYKYQNDDVPKHIKNKRLQY LMELQKRINLEENEKYLEQEVVIIQEGKAGKNGTYMGRTMNNKLIIFESNEELNGEFLKV KVNKITPGPLYGEVVNNLY
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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