Request QuoteCatalog Number: xP429966PZCSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP429966PZCYeast1mgQuote
EP429966PZCE. coli1mgQuote
BP429966PZCBaculovirus200ugQuote
MP429966PZCMammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain MIT 9211)
UniProt IDA9BBS2
Gene NamemiaB; Locus:P9211_13531
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMLSTKTLPSSKAQESLQSPTKGSYWITTFGCQMNKADSERMAGILERMGYKQAEEELQAD LVLYNTCTIRDNAEQKVYSYLGRQALRKKLFPQLKLIVAGCVAQQEGESLLRRVPELDLV MGPQHANRLETLLNKVETGQQVLATEELYIIEDLTTARRDSSICAWVNVIYGCNERCTYC VVPSVRGKEQSRTPPAIKREIENLANTGFKEVTLLGQNIDAYGRDLPGTTPQGRKKNTLT DLLYYIHDIEGIERIRFATSHPRYFTSRLIEACAELPKVCEHFHIPFQSGDDEILNQMRR GYKIKTYKKIIGRIRELMPNASITADVIVAFPGETNEQFQRSLSLVEEIGFDQLNTAAYS PRPNTPAALWPNQIPEEIKIERLRKLNSLVEIKAKERNVRYKDRVEEVLAENTNPKDKQQ LMGRTRTNRLTFFPKVDNQGNSYKPGDLVKVRVKDIRAFSLSGVIEN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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