Request QuoteCatalog Number: xP431841GENSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP431841GENYeast1mgQuote
EP431841GENE. coli1mgQuote
BP431841GENBaculovirus200ugQuote
MP431841GENMammalian Cell200ugQuote

Protein Information

SpeciesGluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)
UniProt IDA9HIM2
Gene NamemiaB; Locus:GDI1843, Gdia_0070
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-480
Expression Tag6xHis
Purity>90%
AA SequenceMVRRRAQEVSAVTDQPACPPAMDLAPASGSRGLHVITWGCQMNVYDSARMTDVLRPLGYH PVDTPDTADMVILNTCHIRDRAAEKVFSELGRLRLVKEARATEGQQTVLAVAGCVAQAEG KEILARAPFVDIVLGPQTYHRLPEMVARAARAAGAVIDTDFPAEQKFDFLPDAQAPQSPG GITSFLTIQEGCDKFCSFCVVPYTRGAEASRPVASVLREARRMVECGAREITLLGQNVNA YHGEGPDGRVWGLARLAEALAAIPGLARIRYTTSHPRDMDADLIAAHRDLPALMPFLHLP VQSGSDRILDAMNRGHTAADYRDLVLRLRDARPDIALSSDFIVGHPGETDADFEATLQLI RDVGFAQAFSFKYSPRPGTPAAGAPLQVAEDVKDARLQALQALLRTQQDAFNDGTVGHVV PVLFTGHGRKAGQLSGRSPYLQPVHVEGPDSLIGQIANVEIRERYTNSLSGTLVQERAFA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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