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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP435584BPHYeast1mgQuote
EP435584BPHE. coli1mgQuote
BP435584BPHBaculovirus200ugQuote
MP435584BPHMammalian Cell200ugQuote

Protein Information

SpeciesBrucella canis (strain ATCC 23365 / NCTC 10854)
UniProt IDA9M9Y3
Gene NamemiaB; Locus:BCAN_A2196
Protein Name
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMSDDTTQIEPAMAQETSPRANTRKVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDA DLVLLNTCHIREKASEKLYSALGRLRKMKDARAADGKELTIGVAGCVAQAEGQEILRRAP NVDLVIGPQTYHRLPNALARVRGGEKVVETDYAIEDKFEHLPAPRREETRKRGVSAFLTV QEGCDKFCTFCVVPYTRGSEVSRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDG REWGLGELLFRLARIPGIARLRYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRI LKAMNRRHKADEYLRLIERIRNVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQA YSFKYSPRPGTPGADLDDHVEEAVKDERLQRLQALLSAQQYAFQDSMIGRKMDVLLEKPG REAGQMVGRSPWLLPVIIDDNKDRVGDIIHVKIVSTGTNSLIAQKLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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