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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP535405DJMYeast1mgQuote
EP535405DJME. coli1mgQuote
BP535405DJMBaculovirus200ugQuote
MP535405DJMMammalian Cell200ugQuote

Protein Information

SpeciesDesulforudis audaxviator (strain MP104C)
UniProt IDB1I241
Gene NamemiaB; Locus:Daud_0549
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-443
Expression Tag6xHis
Purity>90%
AA SequenceMEMERKFVHTITFGCQMNEFDSELMTGLLEGMGYEPAKSLREADLVLINTCCVRESAENR VWGLLGSLKRYKRDKPELIVAVSGCLPQQEGTAEEIIRRFPVVDLVLGTHNRHELPGLIE EVRAGRRPVLGVRQPDSAVPEGLPVRRKSGLRAWVPVIHGCNNFCTYCVVPYVRGRECSR RPDAVVDEVCGLAAAGYREVTLLGQNVNSYGRDLGEGIDFAALLARLDGVEGLWRIRFTT SHPRDFTDRLIEVVARAAKVCEHIHLPAQAGSNRVLQRMNRGYTREDYLDLVARIRAAVP DVSLTTDLMVGFPGETEEDFADTLDLVRRVGYDQAFTFVYNPRRGTPAAGWPDQVPEDVK SRRIQELIQVQKEIGLARNRAEEGKVLEVLVEGPSATRPDLLSGRSRTNKTVVFPGEPGL AGQLVRVRVEVGHLTYLAGRVED
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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