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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP532156TMIYeast1mgQuote
EP532156TMIE. coli1mgQuote
BP532156TMIBaculovirus200ugQuote
MP532156TMIMammalian Cell200ugQuote

Protein Information

SpeciesThermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum)
UniProt IDB0K9L4
Gene NamemiaB; Locus:Teth39_1173
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-471
Expression Tag6xHis
Purity>90%
AA SequenceMPTNIYVTEEELKKQEKIMKEIAEENKGKNLYYHIETYGCQMNVHDSEKLAGMLEKMGYK YTENLEQADVLLFNTCAVREHAEIRVLGRVSQMKELKARNPNLIIGVSGCMMQEKNVVEA IKEKYSYIDIVFGTHNIYKFPQLLWEALNSQDIVIDIIEDTKNVIEELPVKRDSNLKAWV NIIYGCNNFCTYCIVPYTRGREKSRKPEDIIAEVKELAQKGYKEITLLGQNVNSYGKDLD EDITFAKLLYKLNDIEGIERIRFMTSHPKDISDELIYAIRDLDKVCEHLHLPVQAGSNKI LKKMNRKYTKEHYLEIIDKVRSNIPDIAITTDIIVGFPGETEEDFLETLDLVERVRFDAA YTFIYSKRAGTVAANMPDQVDDAVKHERLERLIELQNKISLEKSAELRGKIVEVLIEGIS KRDSNKLTSRTRTNKVVHFVGDESLIGKLANVKITETKAWTMQGELVEVIR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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