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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP431958CWZYeast1mgQuote
EP431958CWZE. coli1mgQuote
BP431958CWZBaculovirus200ugQuote
MP431958CWZMammalian Cell200ugQuote

Protein Information

SpeciesClostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
UniProt IDA9KMU9
Gene NamemiaB; Locus:Cphy_2599
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-456
Expression Tag6xHis
Purity>90%
AA SequenceMALCKAEVKKVADELGRPLTFNVQTFGCQMNAKDSEKLAGILETIGYVESDSEEADFVVY NTCTVRENANTRVYGRIGFLGNLKKKNPHMRIALCGCMMQESHVVEKIKKSYRFVDIVFG THNIFKLAELIYARHTTKKMVIDIWKETDKIVEELPSEQKYKFKAGVNIMYGCNNFCSYC IVPYVRGRERSRNPEDIIKEIKQLVSKGVVEVMLLGQNVNSYGKTLDEPVSFAQLLQMVE QVEGLKRIRFMTPHPKDLSNDVIEVMKNSKKICNHIHLPVQSGSTELLMKMNRKYTKEDY LNLVDRIKMAMPNISLTTDIIVGFPGETEEDFLDTLDVVRKVGYDSAYTFIYSKRSGTPA ATMENQIPEEVANERFQRLLTTIRESSSKISKDDEGKIAEVLVEEVNEQDNSLMTGRLSN NVLVHFKGTKELIGNIVSVKLSECKGFYYMGEMMED
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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