Request QuoteCatalog Number: xP413044ODHSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP413044ODHYeast1mgQuote
EP413044ODHE. coli1mgQuote
BP413044ODHBaculovirus200ugQuote
MP413044ODHMammalian Cell200ugQuote

Protein Information

SpeciesOchrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168)
UniProt IDA6WWX6
Gene NamemiaB; Locus:Oant_0758
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-462
Expression Tag6xHis
Purity>90%
AA SequenceMSDNITDLEPTAERPNVRKVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLL NTCHIREKASEKLYSALGRLRKMKDAREANGKELTIGVAGCVAQAEGQEILRRAPNVDLV IGPQTYHRLPNALARVRSGEKVVETEYALEDKFEHLPSPKREETRKRGVSAFLTVQEGCD KFCTFCVVPYTRGSEVSRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGAGDDGREWGL GELLFRLARIPGIARLRYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMN RRHKADEYVRLIERIREVRPDLALSGDFIVGFPGETDQDFEDTMRLVRDVNYAQAYSFKY SPRPGTPGADLDDHVEEAVKDERLQRLQALLSEQQYAFQDSMIGREMDVLLEKPGRVAGQ MVGRSPWLLPVIIDDSNDRVGDIIHVKITSTGTNSLIAQKLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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