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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP409608KAWYeast1mgQuote
EP409608KAWE. coli1mgQuote
BP409608KAWBaculovirus200ugQuote
MP409608KAWMammalian Cell200ugQuote

Protein Information

SpeciesKineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)
UniProt IDA6W848
Gene NamemiaB; Locus:Krad_1499
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-510
Expression Tag6xHis
Purity>90%
AA SequenceMSTTTATPAGADDTVAAARTYQVRTFGCQMNVHDSERLSGLLEDAGYVRFDEAGRPEGSD APVEPDVVVFNTCAVRENADNKLYGNLGHLAPVKERRPGMQIAVGGCLAQKDRGEIVRKA PWVDVVFGTHNVGSLPVLLERARHNAEAQVEILESLETFPSTLPTRRESPYAAWVSISVG CNNTCTFCIVPALRGKEKDRRPGDVLAEIEALVGEGVLEVTLLGQNVNTYGVEFGDKLAF GKLLRATGGIEGLERVRFTSPHPSSFTDDVVDAMAETPNVMPSLHMPLQSGSDRVLKAMR RSYRQSRFLGIIDRVRSSIPDAAITTDIIVGFPGETDEDFEQTLHVVEQARFSSAFTFQY SPRPGTPAATMGDQIPKRVVQERYERLTALQDRITYEDNQAQTGRTLEVLVAEGEGRKDA ATRRLSGRAPDNRLVHFALPEGAQAPRPGDVATVTVTRGAPHYLEADDVSGFAVRRTRAG DAWEARQARPEPESTGPRPVGLGLPTLRRA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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