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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP535349UBJYeast1mgQuote
EP535349UBJE. coli1mgQuote
BP535349UBJBaculovirus200ugQuote
MP535349UBJMammalian Cell200ugQuote

Protein Information

SpeciesUncultured termite group 1 bacterium phylotype Rs-D17
UniProt IDB1H0D2
Gene NamemiaB; Locus:TGRD_481
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-418
Expression Tag6xHis
Purity>90%
AA SequenceMNYLIETIGCQMNVCDSDMLVSIFSAYGASKANNLSEADVVILNTCSVRSQAEQKAFSYL GRVKEFKQKNPCIKIVVIGCMAERLGPNIKKRFSSVDLIIGAKDIGNAALKIMNLFRTDY SAKKVNSEIKSKIVRYITIMRGCDNYCSYCAVPFVRGREVSINCETIVNECSSMVKNGAR EIILLGQNVNSYQYEDVNFASLIKKTAAIENLERIRFMTNHPKDLSDDLIKIMATEPKVC PHIHIPMQSASDKILKAMNRKYSYEHYLGLIKKLRTAVPDVSVTTDIIVGFPGETDEDFE DTLKAVKTIRFGGLYVFRYSPRPDTKAAEMIDDVPFEEKKRRHAVVLKESNKISIEIVSE MLGSTQQVLAEEIKNGIIKARTKNGRKVFAEGRKEYIGKHINVNIKEAKINSLFGDIV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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