Request QuoteCatalog Number: xP425583STRSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP425583STRYeast1mgQuote
EP425583STRE. coli1mgQuote
BP425583STRBaculovirus200ugQuote
MP425583STRMammalian Cell200ugQuote

Protein Information

SpeciesShewanella sediminis (strain HAW-EB3)
UniProt IDA8FZ02
Gene NamemiaB; Locus:Ssed_3471
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLMDEYEGYTLTEEASEADVLLLNTCSIREKAQEKVF HQLGRWKKLKEKNPDLIIGVGGCVASQEGKVIKERAQCVDLIFGPQTLHRLPEMIDQIRD GGKAVIDISFPEIEKFDKLPEPRADGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD IILEIAQLADQGVREVNLLGQNVNAYRGATHDDEICTFAELLRYVAAIDGIDRLRFTTSH PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTQMKRGHMAIEYKSIIRRLRKARPDI QISSDFIIGFPGESKQDFADTMKLIEDVQFDHSFSFIYSARPGTPAADLPDDVTLAEKKE RLAILQDRITQQAMRYSRQMLGTVQRILVEGPSVKNPMELRGRTETSRVVNFEADPKHIG SFVDVEIVDVYTNSLRGTFVRGEDEMDLRRSLTPSDILAKHKKDDDLGVTQYIP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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