Request QuoteCatalog Number: xP423425BOLSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP423425BOLYeast1mgQuote
EP423425BOLE. coli1mgQuote
BP423425BOLBaculovirus200ugQuote
MP423425BOLMammalian Cell200ugQuote

Protein Information

SpeciesBacillus pumilus (strain SAFR-032)
UniProt IDA8FDH0
Gene NamemiaB; Locus:BPUM_1605
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-508
Expression Tag6xHis
Purity>90%
AA SequenceMNEEQRKASGQVSSSDKKSEKDYSQYFEAVYIPPSLKDAKKRGKEEVEYNQFQIDERFQG LGNGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEPTNSTEDANVILLNTCAIRENAENKV FGELGHLKALKREKPDLILGVCGCMSQEESVVNRILKKHPFVDLIFGTHNIHRLPELLSE CYLSKEMVIEVWSKEGDVIENLPRARQGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSRR PDEIIQEVRRLAAEGYKEITLLGQNVNAYGKDFEDMEYGLGHLMDELRKIDIPRIRFTTS HPRDFDDHLIEVLAKGGNLLDHIHLPVQSGSSSVLKLMARKYDRERYLDLVRKIKEAMPN ASLTTDIIVGFPNETDEQFEETLSLYREVEFDAAYTFIYSPREGTPAAKMQDNVPMHVKK ERLQRLNALVNEISAKKMKEYEGQTVEVLVEGESKNNPEILAGYTSKSKLVNFKAPKEAI GNIVKVKITQAKTWSLDGEMVGEAIEVN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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