Request QuoteCatalog Number: xP428410DHWSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP428410DHWYeast1mgQuote
EP428410DHWE. coli1mgQuote
BP428410DHWBaculovirus200ugQuote
MP428410DHWMammalian Cell200ugQuote

Protein Information

SpeciesDesulfococcus oleovorans (strain DSM 6200 / Hxd3)
UniProt IDA8ZVH2
Gene NamemiaB; Locus:Dole_2355
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-466
Expression Tag6xHis
Purity>90%
AA SequenceMKRFYIHTIGCQMNVYDSSQLSAILTAMGHRSVNAPEQADLVFVNTCTIRAKAKQKATSF VGRLAAMKRARPDMIVGVGGCLAQEEGRQLLDAFPCVDIVFGTHALGRLPGHIQAVAHQG DRIVDVEMTAAIDESVHALQGPDSSGVTGFITIMRGCDNFCTYCVVPYVRGRETSRAPEH ILDEIRARVAGGLREITLLGQNVNSYGQKEGLCSFADLLARVNEIDGLHRIRFTTSHPKD LSPELAAAFTSLDKLCSHVHLPAQSGSDAVLKRMNRRYTRQAYLEKLHWLREAQPGMALS TDIIVGFPGETEQDFLQTLDLIEKVRYDSIFAFMYSDRPLAPARAFDGKVDEAEKQQRIY ALLELQNRITAEKNRALEGRVEQVLVEGKSKSSGRNDITADTVQWTGRTTCNRVANFTVP RELASGNAVGPGAMVRVEIMSGLAHSLSGIAVGVEKPGAGGDAHAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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