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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP428738SUXYeast1mgQuote
EP428738SUXE. coli1mgQuote
BP428738SUXBaculovirus200ugQuote
MP428738SUXMammalian Cell200ugQuote

Protein Information

SpeciesSulcia muelleri (strain GWSS)
UniProt IDA8Z642
Gene NamemiaB; Locus:SMGWSS_194
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-453
Expression Tag6xHis
Purity>90%
AA SequenceMIKKKFYIENYGCQMNISDSEIVSSILNNKGFIKTENLKEANIILINTCSIRDKSEKKIL LRINQIKFIIKKNNDILIGILGCMAYKFKNIKEKKLINLVVGPDSYREIPNLINNFFKKK GEYISTSFSKTETYADIIPKREEKKITAFVTIMRGCDNMCTFCVVPFTRGREKSRDPYSI IKECKFLFKKGYKEIILLGQNVDSYLWYGGGLKKKFKIKEIKNEEIINFSKLLELVAISV PLMRIRFCTSNPNDMSDNVLNVIKKYINICKHIHLPVQSGSNRILSLMNRKHTCEDYILL INKIKNIIPNCSLSCDIITGFCNENENDHNETLNLMNYVKYNFSYMFIYSHRIGTYAYKK LIDNVSLSTKKRRLTEIINLQKTHSYYRNRKYIGSIQDILIEGISTKNINFFYGRNSGND IVIFPKKNYKIGDFIKVKINNCTSATLVGDIYV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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