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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP533562XAZYeast1mgQuote
EP533562XAZE. coli1mgQuote
BP533562XAZBaculovirus200ugQuote
MP533562XAZMammalian Cell200ugQuote

Protein Information

SpeciesXanthomonas campestris pv. campestris (strain B100)
UniProt IDB0RRW2
Gene NamemiaB; Locus:xcc-b100_1844
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-485
Expression Tag6xHis
Purity>90%
AA SequenceMPGTSVSDLPTTATAVDAPALLPLPVGRPQAPALVRGKLYIKTHGCQMNEYDSAKMADVL AASEGLELTDNPEDADVVLVNTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVAS QEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDISFPEIEKFDRLPEPRADG PSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAY RGAYGADAGEPAQYADLGLLIRTIAQIDGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANY LHLPVQAGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIVGFPGETDADFDKT MKLIEDVGFDQSFSFIYSRRPGTPASDLEDDTPDAVKQARLARLQAHINAHAAGISQRMV GSVQRVLVEGPSRRDANELTGKTENMRPVNFPGNPRLVGQFVDVLITEALSNSLRGRIQL DDSAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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