Request QuoteCatalog Number: xP534786DTVSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP534786DTVYeast1mgQuote
EP534786DTVE. coli1mgQuote
BP534786DTVBaculovirus200ugQuote
MP534786DTVMammalian Cell200ugQuote

Protein Information

SpeciesClavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / JCM 9667)
UniProt IDB0RGZ1
Gene NamemiaB; Locus:CMS1210
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-530
Expression Tag6xHis
Purity>90%
AA SequenceMSTVAEHVRAAPSSVDRPRTYEVRTYGCQMNVHDSERLTGSLEAAGYVSAEGAEADIVVI NTCAVRENADNKLYGNLGHLAGVKRRHEGMQIAVGGCLAQKDRATVLEKAPWVDVVFGTH NMGALPTLLERARHNGEAQLEILESLETFPSTLPTKRDEIASGWVSISVGCNNTCTFCIV PALRGKEKDRRPGDILAEIQALVDDGAVEVTLLGQNVNSYGVEFGDRQAFGKLLRAAGAI EGLERIRFTSPHPAAFTDDVIDAMAETPAVMPQLHMPLQSGSDRILKAMRRSYRSERFLG ILDRVRTRVPDAAITTDIIVGFPGETEEDFQETLRVVEAARFSSAFTFQYSIRPGTPAAT MEEQVPADVVKERYGRLTALQERISHEENQRVVGRTVEVLVSAHEGRKDGDTRRVTGRAQ DGRLVHLDVPEGSAEPRPGDAVDVEVTRAAPFHLIADSVDGAPLRIRRTRAGDAWERAQA DSCGVPTPATGASAGAAPRVSLGLPTLRVPTTASTSAPVGDGSAHPRHRA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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