Request QuoteCatalog Number: xP541224SYYSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP541224SYYYeast1mgQuote
EP541224SYYE. coli1mgQuote
BP541224SYYBaculovirus200ugQuote
MP541224SYYMammalian Cell200ugQuote

Protein Information

SpeciesShewanella woodyi (strain ATCC 51908 / MS32)
UniProt IDB1KDV4
Gene NamemiaB; Locus:Swoo_3696
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLMDEYKGYTLTEEAEEADVLLLNTCSIREKAQEKVF HQLGRWKKLKDKNPNLIIGVGGCVASQEGKVIKDRAQCVDLIFGPQTLHRLPEMIDQIEA GGKAVIDVSFPEIEKFDRLPEPRADGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD VILEIAQLAEQGVREVNLLGQNVNAYRGATHDDEICTFAELLRYVAAIDGIDRLRFTTSH PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTQMKRGHMAIEYKSIIRRLRKARPDI QVSSDFIIGFPGESKQDFEDTMKLIEDVQFDHSFSFIYSARPGTPASDLPDDVTLDEKKE RLAILQDRITQQAMRYSRQMLGTVQRILVEGPSVKNPMELRGRTETSRVVNFEADPKHIG SFVDVEIVDVYTNSLRGNFIRGEDEMDLRRSLRPSDILAKHKKDDELGVTQYIP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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