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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP543858BXQYeast1mgQuote
EP543858BXQE. coli1mgQuote
BP543858BXQBaculovirus200ugQuote
MP543858BXQMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia cenocepacia (strain MC0-3)
UniProt IDB1JY57
Gene NamemiaB; Locus:Bcenmc03_2721
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-457
Expression Tag6xHis
Purity>90%
AA SequenceMTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRA SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTDIADFATLIEYVADIPGIERIRYTT SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP DLSLSTDMIVGFPGETEDDFDKMMALVHEMGYDTSFSFIYSPRPGTPAANLADDTPREVK LKRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLAS HPRLIGQMIDVKINHAYPHSLRGELVLVSDDASTATH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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