Request QuoteCatalog Number: xP541110YAXSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP541110YAXYeast1mgQuote
EP541110YAXE. coli1mgQuote
BP541110YAXBaculovirus200ugQuote
MP541110YAXMammalian Cell200ugQuote

Protein Information

SpeciesYersinia pseudotuberculosis serotype O:3 (strain YPIII)
UniProt IDB1JG95
Gene NamemiaB; Locus:YPK_3005
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVF SLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQE TNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDD ILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRIRFTTSH PIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDI QISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQ RLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIG KFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIYQP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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