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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP423758RIMYeast1mgQuote
EP423758RIME. coli1mgQuote
BP423758RIMBaculovirus200ugQuote
MP423758RIMMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia bellii (strain OSU 85-389)
UniProt IDA8GY24
Gene NamemiaB; Locus:A1I_07380
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLYIKTYGCQMNVYDSVKMQDLLYPYGYEPTENIEEADVIILNTCHIREKAAEKTYS ELGRIKKLQDARKKQGLNSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELIS KVVRHEKHLIDLDFVEEAKFDQLPEQLYPQGASSFISVQEGCDKFCTFCVVPYTRGAEFS RNVEQVYREALKVVSSGAKEIMLLGQNVNAYHGKTSDDKVFTLADLIRHLVKIPNLERLR YTTSHPIDMTDDLISLHGLEPKLMPFLHLPVQSGSNKTLKAMNRKHDRDYYFDIIDRLRK ARADIVLSSDFIVGFPGETDEDFADTLDLVRKVKYGQCYSFKYSPRPGTPGATRTDQVPE HIKSERLTILQKELAAQQLAFNESCIGSTMKVLFDRDGKFDDQIIGKTPYMQSVYIKNPN KDLLGKIIEVKITKAALNSLSGEIYR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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