Request QuoteCatalog Number: xP415436BOKSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP415436BOKYeast1mgQuote
EP415436BOKE. coli1mgQuote
BP415436BOKBaculovirus200ugQuote
MP415436BOKMammalian Cell200ugQuote

Protein Information

SpeciesBacillus cereus subsp. cytotoxis (strain NVH 391-98)
UniProt IDA7GRA2
Gene NamemiaB; Locus:Bcer98_2424
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-509
Expression Tag6xHis
Purity>90%
AA SequenceMNEQQRLASQQANASTKKEEKDYSKYFEHVYQPPSLKDAKKRGKEQVKIERDFGLPEEFR NFGKGRKFYIRTYGCQMNEHDTEVMAGIFTTLGYEPTFTTEDADVILLNTCAIRENAENK VFGELGHLKPLKQKNPDLLIGVCGCMSQEESVVNKIMQKHQHVDMVFGTHNIHRLPYILK DAMFSKATVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR RPEDIIKEVRHLAANGYKEITLLGQNVNAYGKDFDDLEYGLGDLMDELRKIDIARIRFTT SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYTREQYLELVRKIKKTIP NVVLTTDIIVGFPNETDEQFEETLSLYREVEFDSAFTFIYSPREGTPAAKMKDNIPMEVK KERLQRLNELVNEFSAKKNKKYEGQIVEVLVDGESKNNPDVLAGYTRTNKLVNFVAPKSV IGQLVKVKITEAKTWSLNGELVEEPIEVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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