Request QuoteCatalog Number: xP421812DIBSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP421812DIBYeast1mgQuote
EP421812DIBE. coli1mgQuote
BP421812DIBBaculovirus200ugQuote
MP421812DIBMammalian Cell200ugQuote

Protein Information

SpeciesDinoroseobacter shibae (strain DFL 12)
UniProt IDA8LSE7
Gene NamemiaB; Locus:Dshi_1019
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-437
Expression Tag6xHis
Purity>90%
AA SequenceMADPKKLYIKTYGCQMNVYDSERMAEAMGGEGYVQTDRAEDADMILLNTCHIREKAAEKV YSELGRFKPLKAEKPDLKIGVAGCVAQAEGAEIMRRQPLVDLVVGPQAYHRLPELTARAA TGAKALDTDFPEEDKFDHLAARPKAKRGPTAFLTVQEGCDKFCAFCVVPYTRGAEVSRPA ARVLTEARDLVERGVREITLLGQNVNAYHGHARGLAGLIWDLAEIDGLERIRFTTSHPND MDDALIAAHGACDKLMPYLHLPVQSGSDRILKAMNRKHTAESYIRLIERIRAARPDILLS GDFIVGFPGETDQDFADTMALVEAVGYGQAYSFKYSARPGTPAAEKEDVPGEVADARLQT LQALLTRQQRAIQDAKVGTTARVLFEKPGRLPGQMVGKSEHLHAVHVAAPDAARGDLVRV RIAESSANSLRGVLIAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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