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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP426862STPYeast1mgQuote
EP426862STPE. coli1mgQuote
BP426862STPBaculovirus200ugQuote
MP426862STPMammalian Cell200ugQuote

Protein Information

SpeciesShewanella pealeana (strain ATCC 700345 / ANG-SQ1)
UniProt IDA8H7B6
Gene NamemiaB; Locus:Spea_3137
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLLDEYEGYTLTDNAEEADVLLLNTCSIREKAQEKVF HQLGRWKTLKDKKPGLIIGVGGCVASQEGKAIKERAQCVDLIFGPQTLHRLPEMIDQIKD GKKAVIDVSFPEVEKFDRLPEPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD VILEIAQLAEQGVREVNLLGQNVNAYRGATHDDEICTFAELLRYVAAIDGIDRLRFTTSH PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDLILTQMKRGHMAIEYKSIIRRLRKARPDI LISSDFIIGFPGESKQDFADTMKLIEDIQFDHSFSFIYSARPGTPAADLPDNVSLDEKKE RLAILQDRITQQAMRYSRQMVGTVQRILVEGPSVKNPMELRGRTENSRVVNFEGLHEHIG KFVDVEIVDVYTNSLRGVFIRGEDEMDLRRDLRPSDITAKYKQADDLGVTLFTPA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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