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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP377369MOQYeast1mgQuote
EP377369MOQE. coli1mgQuote
BP377369MOQBaculovirus200ugQuote
MP377369MOQMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium vanbaalenii (strain DSM 7251 / PYR-1)
UniProt IDA1T7U2
Gene NamemiaB; Locus:Mvan_2429
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-510
Expression Tag6xHis
Purity>90%
AA SequenceMTSTVTQQASGPSPDRSARTFEVRTYGCQMNVHDSERLAGLLEGAGYRRAGEGADADIVV FNTCAVRENADNKLYGNLSHLAPRKQSDPDMQIAVGGCLAQKDRDSVLRKAPWVDVVFGT HNIGSLPALLDRARHNRVAQVEIAEALQEFPSALPASRESSYAAWVSISVGCNNTCTFCI VPALRGREVDRRPGDVLAEVQSLVDQGVLEITLLGQNVNAYGVSFADPTEPRDRGAFAKL LRACGRIDGLERVRFTSPHPAEFTDDVIEAMAETPNVCPTLHMPLQSGSDRILRAMRRSY RAERYLGIIDRVRAAIPHAAITTDLIVGFPGETEEDFQATLDVVAASRFSSAFTFQYSKR PGTPAAELADQVPKAVVSERYQRLIELQERISLEENTAQIGRRVELLVATGEGRKDAATA RMSGRARDGRLVHFAPGAVGSEVRPGDVVVTTVTGAAPHHLIADAGLIEHRRTRAGDAHA AGQKPRTGVGLGMPAVGAPDPLPATTGCAR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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