Request QuoteCatalog Number: xP393252CXGSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP393252CXGYeast1mgQuote
EP393252CXGE. coli1mgQuote
BP393252CXGBaculovirus200ugQuote
MP393252CXGMammalian Cell200ugQuote

Protein Information

SpeciesCorynebacterium glutamicum (strain R)
UniProt IDA4QEV7
Gene NamemiaB; Locus:cgR_1779
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-526
Expression Tag6xHis
Purity>90%
AA SequenceMTQQLNHAKVNQHPGQATLPETAEGQVRTYEVKTYGCQMNVHDSERLSGLLEEAGYVAAP EDATPDLVVFNTCAVRENADMRLYGTLGNLRSVKEKNPGMQIAVGGCLAQKDKDTVVKKA PWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIVDSLEQFPSVLPAKRESAYAGWVSVSVG CNNTCTFCIVPSLRGKEQDRRPGDILAEVQALVDQGVTEVTLLGQNVNAYGVNFVDPELE RDRSAFSKLLRACGEIEGLERVRFTSPHPAEFTSDVIDAMAETPNICPQLHMPLQSGSDK VLKEMRRSYRSKKFLSILDEVRAKIPHASITTDIIVGFPGETEEDFQATLDVVKKARFTS AYTFQYSPRPGTPAAEYENQLPKEVVQERYERLMVVQEQVCEEENQKLIGTTVELLVQAG GGRKNDATKRMSGRARDGRLVHFAPEGDIDGEIRPGDFVTVTVTEAKPFFLIADSGVQTH RRTKAGDNSAVGQVPTTAPIGVGLGLPQIGAPKVAPATESACCSIN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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