Request QuoteCatalog Number: xP392273BPVSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP392273BPVYeast1mgQuote
EP392273BPVE. coli1mgQuote
BP392273BPVBaculovirus200ugQuote
MP392273BPVMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808) )
UniProt IDA4JHN3
Gene NamemiaB; Locus:Bcep1808_2795
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-457
Expression Tag6xHis
Purity>90%
AA SequenceMTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRA SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD VLTEVAGLADQGVREVTLLGQNVNAYRGALSAGSADIADFATLIEYVADIPGIERIRYTT SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP DLSLSTDMIVGFPGETEDDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVK LKRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELSGRTENNRVVNFPAPLAS HPRLIGQMIDVKINHAYPHSLRGELLLVSDDASAATH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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