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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP392186DHXYeast1mgQuote
EP392186DHXE. coli1mgQuote
BP392186DHXBaculovirus200ugQuote
MP392186DHXMammalian Cell200ugQuote

Protein Information

SpeciesDesulfotomaculum reducens (strain MI-1)
UniProt IDA4J5Q8
Gene NamemiaB; Locus:Dred_1889
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-456
Expression Tag6xHis
Purity>90%
AA SequenceMAEKHQDKMTNAMNNSKLYLIQSFGCQMNERDAESLAGMLEDLGYCPTSAQEEADIILLN TCCVRETAESKVFGLLGRLRKLKVAKPDLILGVCGCMSQQEDAAKRIRRSFPFVDLIFGT HNIHELPRMIHQVQENHEAVLEVWATEKGITESIPVKRKDKLKAWVTIMYGCNNFCTYCI VPYVRGRERSRQPEDIIDEIKELVQEGYKEVTLLGQNVNSYGKDFKNNYRFADLLMAIDD ITGLERVRFMTSHPRDFDQRLIEVVASAKKVCEHYHLPAQAGSNRVLKMMNRGYTREHYL ELIRKIKERVPNASITADLMVGFPGETEEDFQETLDLVKQVRYDSAFTFVYNIRSGTPAA KLEQVSEEVKSERIQRLIELQNLISLENNQREEGRVLEVLVEGETKTNPDLLAGRTRTNK LVVFQGSGHLPGQLVQIRITKGRPNLLEGEVVPNGT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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