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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP372881PYCYeast1mgQuote
EP372881PYCE. coli1mgQuote
BP372881PYCBaculovirus200ugQuote
MP372881PYCMammalian Cell200ugQuote

Protein Information

SpeciesParacoccus denitrificans (strain Pd 1222)
UniProt IDA1B8C4
Gene NamemiaB; Locus:Pden_3701
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-445
Expression Tag6xHis
Purity>90%
AA SequenceMSDQASKPAPAVKKLFIKTYGCQMNVYDSQRMAEAMGAEGYVLTENQSDADMVLLNTCHI REKAAEKLYSDLGRLKPLKAERPDLKIGVAGCVAQAEGEEIQRRMPIVDLVVGPQAYHRL PAMARAGRGVDTEFPAEDKFEHLPKPAATRRAPAAFLTVQEGCDKFCAFCVVPYTRGAEV SRPVSRILAEARDLVARGVREITLLGQNVNGWHGEGPEGSEWGFGRLIRAIAEIDGLDRI RYTTSHPNDMADDLIAAHRDEPKLMPYLHLPVQSGSDRILKAMNRRHTVDQYLRLIERIR EARPDIMLTSDFIVGFPGETDQDHQGTLELVRAVNFGTAFSFKYSPRPGTPAYERPEIEG AVADARLQELQALLTSQQKAAQEGMVGRELGVLFEKPGRNPGQMVGKSDYLHAVFVEAPA AKVGDLVRVRITHSAPNSLAGVLAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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