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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP398059BPFYeast1mgQuote
EP398059BPFE. coli1mgQuote
BP398059BPFBaculovirus200ugQuote
MP398059BPFMammalian Cell200ugQuote

Protein Information

SpeciesBradyrhizobium sp. (strain ORS278)
UniProt IDA4YJD7
Gene NamemiaB; Locus:BRADO0041
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMTPPRKLHIKSYGCQMNVYDAQRMVDTLGAEGFVETADAGDADLVILNTCHIREKASEKV YSELGRLRVAKEEAARSGRAMQIAVAGCVAQAEGVEIISRAPTVDVVVGPQSYHHLPQLL AQASRGERAIETEFPAEDKFGFLAKPSREAIRARGVSAFVTVQEGCDKFCTFCVVPYTRG AEMSRPVARIVDDVMQLTDSGVREITLIGQNVNAYHGEGPDGRTWTLGRLLYRIAEIPGV ARIRYSTSHPNDVDDGLIAAHRDLTAVMPFVHLPVQSGSDRILAAMNRKHSAADYRRVVD RFRGARDDIAFSSDFIVGFPGETEEDFRATLALIDQIGYAAAYSFKYSPRPGTPAADMQE MVSATEMDERLERLQSLIDSQQAAFNKAAIGSVVDVLFEREARKPGQLVGRTAYLQPAHV MASSDIIGQVLPVRIDSLERYSLLGELVATTAAPIPEAAMPLASIGG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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