Request QuoteCatalog Number: xP397817STQSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP397817STQYeast1mgQuote
EP397817STQE. coli1mgQuote
BP397817STQBaculovirus200ugQuote
MP397817STQMammalian Cell200ugQuote

Protein Information

SpeciesShewanella putrefaciens (strain CN-32 / ATCC BAA-453)
UniProt IDA4Y9E1
Gene NamemiaB; Locus:Sputcn32_2855
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEDASEADILLLNTCSIREKAQEKVF HQLGRWKTLKDKNPNLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPDMIEQVRR GDKAVIDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD IILEIAQLAEQGVREVNLLGQNVNAYRGATHDGSICSFAELLRFVAAIDGIDRIRFTTSH PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKAREGI QISSDFIIGFPGETKEDFADTMKLIEEIGFDHSFSFIYSARPGTPAADLPDNVDMEEKKQ RLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGLPKHIG TFVDVEIVDVYTNSLRGKFIRGEDEMDLRRSLRPSDILAKHKQDDDLGVTQFKP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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