Request QuoteCatalog Number: xP397158PZMSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP397158PZMYeast1mgQuote
EP397158PZME. coli1mgQuote
BP397158PZMBaculovirus200ugQuote
MP397158PZMMammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas mendocina (strain ymp)
UniProt IDA4XYW1
Gene NamemiaB; Locus:Pmen_3779
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-447
Expression Tag6xHis
Purity>90%
AA SequenceMTKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTEKPEEADVILLNTCSIREKAQDKVF SQLGRWRELKQANPDLVIGVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAART TKTAQVDISFPEIEKFDRLPEPRVDGPSAFVSVMEGCSKYCTFCVVPYTRGEEVSRPLAD VLLEITQLTDKGVKEVTLLGQNVNGYRGATPDGRIADFAELLHAVAALDGIERIRYTTSH PLEFSDAIIAAHAEIPQLVKYLHLPVQSGSDRILAAMKRNHTALEYKSRIRKLRAAVPDI LISSDFIVGFPGETEKDFEQTMKLIEDVGFDFSFSFIYSSRPGTPAADLVDDTPDEVKKQ RLAILQHRINQYGFENSRRMVGTVQRILVSDYSKKDPGMLQGRTEQNRIVNFRCDNPRLI GQFVDVHIDDALPHSLRGTLLDASTVN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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