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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP367290STSYeast1mgQuote
EP367290STSE. coli1mgQuote
BP367290STSBaculovirus200ugQuote
MP367290STSMammalian Cell200ugQuote

Protein Information

SpeciesShewanella sp. (strain ANA-3)
UniProt IDA0KTX4
Gene NamemiaB; Locus:Shewana3_1008
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVF HQLGRWKTLKDKNPDLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPDMIDQVRR GEKAVIDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPSDD IILEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICTFAELLRYVAAIDGIDRIRFTTSH PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDI QISSDFIIGFPGETKEDFADTMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVDMEEKKQ RLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGQPKHIG SFVDVEIVDVYTNSLRGKFIRGEDEMDLRRNLRPSDILAKHKQDDDLGVTQFKP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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