Request QuoteCatalog Number: xP376514NFCSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP376514NFCYeast1mgQuote
EP376514NFCE. coli1mgQuote
BP376514NFCBaculovirus200ugQuote
MP376514NFCMammalian Cell200ugQuote

Protein Information

SpeciesNocardioides sp. (strain BAA-499 / JS614)
UniProt IDA1SNG0
Gene NamemiaB; Locus:Noca_3847
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-496
Expression Tag6xHis
Purity>90%
AA SequenceMSTAQHTSPRTYEVRTYGCQMNVHDSERLTGLLEDAGYVAAPDGQQADVVVFNTCAVREN ADNKLYGNLGHLAPVKAATPGMQIAVGGCLAQKDRDTITTRAPWVDVVFGTHNIGSLPAL LERARVQDEAQVEILESLEVFPSTLPTRRESAYAAWVSVSVGCNNTCTFCIVPSLRGKEK DRRPGEILAEVEALVADGVSEITLLGQNVNAYGVEFGDRQAFSKLLRACGEVDGLERVRF TSPHPAEFTDDVIAAMAETPNVMPQLHMPLQSGSDKVLRDMRRSYRQRKFLGILERVRAA MPDAAITTDIIVGFPGETEEDFLQTMHVVRAARFSGAFTFQYSKRPGTPAATLPDQVPPE VVKDRYERLVALVNEIAWEENKRLVGRRVELMVAEGEGRKDAATHRLSGRGPDNRLVHFA PPAGVEIRPGDLATVEVTYAAPHHLVADGPVVDVRRTRSGDAWAARNAAPAPSSGVTLGM PTVGAPAPLPDAPACR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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