Request QuoteCatalog Number: xP399135FDTSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP399135FDTYeast1mgQuote
EP399135FDTE. coli1mgQuote
BP399135FDTBaculovirus200ugQuote
MP399135FDTMammalian Cell200ugQuote

Protein Information

SpeciesFlavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae)
UniProt IDA5FIU2
Gene NamemiaB; Locus:Fjoh_1850
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-481
Expression Tag6xHis
Purity>90%
AA SequenceMEKIIEESKQGESLVLENKPENTKKLFIESYGCAMNFSDSEVVASILSDGGYNTTSVLEE ADLVLVNTCSIRDKAEQTIRKRLEKYNAVKRTNPKMKVGVLGCMAERLKSQFLEEEKIVD LVVGPDAYKDLPNLLAEVEEGRDAINVILSKEETYGDISPVRLMSNGITALVSITRGCDN MCTFCVVPFTRGRERSREPQSIMAEIQDLWSKGFKEITLLGQNVDSYLWYGGGLKKDFVN ASEMQKATAVDFDQLLEMVAVGFPKMRIRFSTSNPQDMHESILHVMAKYPNICKHIHLPV QSGSNRILKEMNRLHSREEYMALIDKIRAIVPDASISQDMIAGFPTETEQDHQDTMSLME YVKYNFGYMYSYSERPGTLAGRKMKDDVEEETKARRLQEIVDLQQKHAWFRSEEFVGKTV EVLVEKVSKKSKDEFSGRNSQSITVVFPKENYKIGDFVNVKITSCTSGTLKGEAVGLSSM N
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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