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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP379005MOMYeast1mgQuote
EP379005MOME. coli1mgQuote
BP379005MOMBaculovirus200ugQuote
MP379005MOMMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium sp. (strain KMS)
UniProt IDA1UEZ3
Gene NamemiaB; Locus:Mkms_2203
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-529
Expression Tag6xHis
Purity>90%
AA SequenceMTSTVTQQAANALPPVARTYQVRTYGCQMNVHDSERLAGLLEDAGYRRAADGADADVVVF NTCAVRENADNKLYGNLSHLAPRKRSEPQMQIAVGGCLAQKDRDAVLRRAPWVDVVFGTH NIGSLPTLLERARHNRAAQVEIAEALQEFPSTLPAARESAYAGWVSISVGCNNTCTFCIV PSLRGKEVDRRPGDVLAEIQTLVDQGVLEVTLLGQNVNAYGVSFAADERLREDPRMWQSV PRNRGAFAELLRACGRIDGLERVRFTSPHPAEFTDDVIEAMAETPNVCPALHMPLQSGSD RILRAMRRSYRAEKYLGIIDRVRAAIPDAAITTDLIVGFPGETEEDFQATLDVVAASRFS SAFTFQYSKRPGTPAADMPGQLPKAVVSERYQRLIELQERISLEENQAQVGRTLELLVAT GEGRKDAATARLSGRARDGRLVHFAPGAAADEPLARRAFDQVRPGDVVTTTVTGAAPHHL IADGALLTHRRTRAGDAHAAGLRPRTGVGLGIPGVGAPAPAPVTTGCAL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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