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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP393505PZJYeast1mgQuote
EP393505PZJE. coli1mgQuote
BP393505PZJBaculovirus200ugQuote
MP393505PZJMammalian Cell200ugQuote

Protein Information

SpeciesProsthecochloris vibrioformis (strain DSM 265) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 265) ) (Chlorobium phaeovibrioides (strain DSM 265) )
UniProt IDA4SCW6
Gene NamemiaB; Locus:Cvib_0303
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-448
Expression Tag6xHis
Purity>90%
AA SequenceMKHEKGRSFYIHTFGCQMNQADSAIITAILMDGGFRVAGSQEDADIVILNTCAVRENAVE RITHQLQFLRGAKRRKKSLLVGVAGCVPQHLKREMLEMFPVIDFLAGPDTYRNLPALIGD AEEGKRPAALEFRIEETYAGIDPLREVGVGAFVPVTRGCNNMCAFCVVPFTRGRERSQPF SAVMDEVRRVVEKGYGEITLLGQNVNSYADPEEGRDFASLLRAVSREAPGCRIRFTTSHP KDISPDLVDAIADSPNICNHVHLPVQSGSSRMLHRMNRGHGIDEYLETVALLRRRIPGVS LTTDLIAGFCGEEDEDHEATLALLREVRYDNAFMFYYSPRFGTAAWKTLADTVPLAVKKQ RLQEIIDLQLSISAECLQEAVGSVVDVLAESESRRSTEQLMGRTGTNRAVVFDRGGARPG DRVSVLISKASSATLTGVNQGVLPAFYS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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