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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP394344EIZYeast1mgQuote
EP394344EIZE. coli1mgQuote
BP394344EIZBaculovirus200ugQuote
MP394344EIZMammalian Cell200ugQuote

Protein Information

SpeciesEnterobacter sp. (strain 638)
UniProt IDA4W838
Gene NamemiaB; Locus:Ent638_1187
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTKKLHIKTWGCQMNEYDSSKMADLLNATHGYELTELAEEADVLLLNTCSIREKAQEKVF HLLGRWKLLKKKNPDLIIGVGGCVASQEGKLIRTRSPYVDIVFGPQTLHRLPEMINSVRG DRSPVVDVSFPEIEKFDRLPEPRADGPSAFVSIMEGCNKYCTYCVVPYTRGEEVSRPCDD ILFEVAQLAAQGVREVNLLGQNVNAWRGENYDGSIGTFAELLRLVAAIDGIDRVRFTTSH PIEFTDDIIDVYRDTPELVSFLHLPVQCGSDRVLNLMGRTHTVLEYKAIIRKLREARPDI QISSDFIVGFPGETTDDFERTMKLIGEINFDVSFSFIFSARPGTPAADMVDDVPEEEKKQ RLYILQERINQQVTAWSRRMLGTTQRILVEGTSRKSVMQLSGRTENNRVVNFEGTPDMIG KFVDIEILEVLTNSMRGKVVRTEDQMGLRVAETPASVIARTRKENDSGAVVYQP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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