Request QuoteCatalog Number: xP405584DUCSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP405584DUCYeast1mgQuote
EP405584DUCE. coli1mgQuote
BP405584DUCBaculovirus200ugQuote
MP405584DUCMammalian Cell200ugQuote

Protein Information

SpeciesClostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum)
UniProt IDA6LWI1
Gene NamemiaB; Locus:Cbei_2555
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-455
Expression Tag6xHis
Purity>90%
AA SequenceMNFDIEKLDSQMASDEKKLFFIQTYGCQMNEEDSEKLSGMLKRMGYENTENRDEASIIIF NTCCVRENAENKVFGNLGALKKQKEKNPDLVIGICGCMMQQKGMADDILKRFPYVNIIFG THNSYKFPEYLNRVKTEGVQIKEIIDKETEIVEGIPIDRKSDIKGFVTIMYGCNNFCTYC IVPYVRGRERSRKPEDIVNEIKDMVTRGYKEVTLLGQNVNSYGKGLEENITFADLLRKVN EIEGLERIRFMTSHPKDLTLDVVYAIRDCDKVCEQIHLPVQSGSDRILKEMNRHYTKEQY ITLAKKIRAEIPDVTFSTDIIVGFPGETEEDFSETLELAKEVRYDAAFTFIYSRRNHTPA DKMENQIPDEIKHERFNRLVEIVNTGIAKGNKDAEGKIYEVLVEGYSKNDEAKLTGRTRN GRLVNFEGGEDLIGKLVNVKIIKANSFSLIGEVEK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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