Request QuoteCatalog Number: xP514820ESQSize: 0.2-1mg

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Recombinant Dihydroxy-acid dehydratase (ilvD)

Recombinant Dihydroxy-acid dehydratase (ilvD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP514820ESQYeast1mgQuote
EP514820ESQE. coli1mgQuote
BP514820ESQBaculovirus200ugQuote
MP514820ESQMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (strain PC1)
UniProt IDC6DHH1
Gene NameilvD; Locus:PC1_4029
Protein NameDihydroxy-acid dehydratase
Region Expressed1-616
Expression Tag6xHis
Purity>90%
AA SequenceMPKYRSATTTHGRNMAGARALWRATGMTDNDFGKPIIAVVNSFTQFVPGHVHLRDLGKLV AEQIEASGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS NCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQIIKLDLVDAMIQGADPKVSDEQ SEQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLNAGKRIV GLAKRYYEQDDESVLPRNIANKAAFENAMILDIAMGGSTNTVLHLLAAAQEGEVDFTMTD IDRLSRQVPHLCKVAPSTQKYHMEDVHRAGGVIGILGELDRAGLLNREVNNVLGKTLPET LEAYDVMLTQDDSVKSMYSAGPAGIRTTQAFSQDCRWDSLDTDRQEGCIRSREFAYSQDG GLAVLYGNLAENGCIVKTAGVDEGSLVFRGPAKVYESQDDAVDAILGGKVVAGDVVVIRY EGPKGGPGMQEMLYPTTYLKSMGLGKSCALITDGRFSGGTSGLSIGHASPEAASGGTIAL VQDGDIIAIDIPNRSIALVLDDNALASRRAAEEARGDQAWTPHNRERQVSFALRAYASLA TSADKGAVRDKSKLGG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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