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Recombinant Cytoplasmic tRNA 2-thiolation protein 2 (ncs2)

Recombinant Cytoplasmic tRNA 2-thiolation protein 2 (ncs2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP003313AVEYeast1mgQuote
EP003313AVEE. coli1mgQuote
BP003313AVEBaculovirus200ugQuote
MP003313AVEMammalian Cell200ugQuote

Protein Information

SpeciesAspergillus niger (strain CBS 513.88 / FGSC A1513)
UniProt IDA2R475
Gene Namencs2; aka: ctu2; ORFs:An14g06820
Protein NameCytoplasmic tRNA 2-thiolation protein 2
Region Expressed1-366
Expression Tag6xHis
Purity>90%
AA SequenceMPGKELPDRCMNCHEAEPAFLLRERHVCQECYIRFLNFKPFRRMERYRLRRNMPQTGPCK LLLPLSYGVSSTVLLHMLHRQLEALRSKKHGPAGFEILVLVVEPSTISPVPPHEEGFALA QQTFPLCSFTRLPFHSIFELDPDVHQIMSQYAGEHFTDDTSLSDEERLNRFRRSITTATS KSDVDQILLNKLVVAFAKKMECRGIVWGDSDSKLAAKTLANVAKGRGSAVTWQVCDGMSP FGLEFNFPLRDVFTAEIRTYATLFPELAGIIVHDEPPSENTLTKNLSIDELMIRYVSTQG EKYPGVMLNVTRTASKLQSSGTSIGGLQCNFCGAFMTTNENITGEQGNEQLQFCYACARS QPELSC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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