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Recombinant Cytochrome P450 4ae1 (Cyp4ae1)

Recombinant Cytochrome P450 4ae1 (Cyp4ae1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP523693DLUYeast1mgQuote
EP523693DLUE. coli1mgQuote
BP523693DLUBaculovirus200ugQuote
MP523693DLUMammalian Cell200ugQuote

Protein Information

SpeciesDrosophila melanogaster (Fruit fly)
UniProt IDO46054
Gene NameCyp4ae1; aka: CYP4E4; ORFs:CG10755
Protein NameCytochrome P450 4ae1
Region Expressed1-496
Expression Tag6xHis
Purity>90%
AA SequenceMLVVLLVALLVTRLVASLFRLALKELRHPLQGVVPSVSRVPLLGAAWQMRSFQPDNLHDK FAEYVKRFGRSFMGTVLGHVVMVTAEPRHIDALLQGQHQLKKGTMYFALRGWLGDGLLLS RGKEWHTMRKIITPTFHFSILEQFVEVFDRQSSILVERLRTLSYGNEVVNIYPLVGLAAL DIITETAMGVNVDAQGADSEVVHAVKDLTNILATRFMRPHLLFPHLFRLCWPSGFRKQQA GVICLHEFTNGIIEQRRRLLAREANQDKPTKPHALLDTLLRATVDGQPLTDKQIRDEVNT FIFEGHDTTTSAVSFCLYLLSRHEAVQQKLFEELRMHYGQDLFRGVILSDFATLPYLSCV VKESLRLYPPIPAVARCLEKDLVIDEGYIPVGTNVVVLLWQLLRDEAIFTDPLVFQPERH LGEEAPRLSPYSYIPFSAGPRNCIGQKFALLEMKTMVTKVIRHYQLLPMGADVEPSIKIV LRSKSGVNVGLRPRLY
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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