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Recombinant D-cysteine desulfhydrase (dcyD)

Recombinant D-cysteine desulfhydrase (dcyD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP300552ENVYeast1mgQuote
EP300552ENVE. coli1mgRP180174Ba
BP300552ENVBaculovirus200ugQuote
MP300552ENVMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain K12)
UniProt IDP76316
Gene NamedcyD; aka: yedO; Locus:b1919, JW5313
Protein NameD-cysteine desulfhydrase
Region Expressed2-328
Expression Tag6xHis
Purity>90%
AA SequencePLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTPMAMGGNKLRKLEFLAAD ALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENPIGTTAENYLTNGNRLLLDL FNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGGSNALGALGYVESALEIAQQCE GAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGVTVSRSVADQLPKVVNLQQAIAKE LELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLARLEGILLDPVYTGKAMAGLIDGISQ KRFKDEGPILFIHTGGAPALFAYHPHV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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